primary rat keratinocytes Search Results


99
ATCC primary epidermal keratinocytes; normal, human, neonatal foreskin
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Cell Applications Inc primary rat keratinocytes
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PromoCell primary normal human epidermal keratinocytes nhek
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Cell Applications Inc cell basal medium
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Cell Applications Inc passage rat epidermal keratinocytes
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
Passage Rat Epidermal Keratinocytes, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lonza keratinocyte basal medium
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
Keratinocyte Basal Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation 004-600
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
004 600, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation atm antibody (2c1)
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
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99
Micromeritics Instrument zetasizer advance
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
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Bio-Techne corporation simple-western-system-0
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
Simple Western System 0, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Dawley Inc primary rat keratinocytes
Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and <t>keratinocytes</t> (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.
Primary Rat Keratinocytes, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and keratinocytes (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.

Journal: Arthritis Research & Therapy

Article Title: Synergistic role of c-Myc and ERK1/2 in the mitogenic response to TGFβ-1 in cultured rat nucleus pulposus cells

doi: 10.1186/ar2567

Figure Lengend Snippet: Effect of transforming growth factor β1 (TGFβ1) treatment on mRNA expression in different cell types (a), Cells were treated with or without 5 ng/mL TGFβ1 for 24 h . The expression of c-myc in nucleus pulposus cells (NP), in articular chondrocytes (AC) and keratinocytes (KT) are presented. The expression of p15 , p21 and p27 in NP was also determined. Time course of c-myc expression in NP treated with 5 ng/mL TGFβ1 (b). The graph shows the relative intensities of c-myc bands normalized for β-actin levels by densitographic analysis. Incubation for 24 h with medium containing various concentrations of fetal bovine serum (FBS) did not alter the level of c-myc expression in NP (c). The reverse transcription-polymerase chain reaction (RT-PCR) was performed on total RNA extracted from the cells. β-actin was used as an internal control.

Article Snippet: Cryopreserved primary passage rat epidermal keratinocytes were obtained from Cell Applications Inc. (San Diego, CA, USA) and maintained in growth medium (Cell Applications Inc.).

Techniques: Expressing, Incubation, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Control